A copper monosulfide-nanoparticle-based fluorescent probe for the sensitive and specific detection of ochratoxin A

The mycotoxin ochratoxin A (OTA) is a secondary metabolite derived from multiple Aspergillus and Penicillium strains. development of rapid, sensitive, simple method for OTA detection important to ensure food biosafety safeguard public health. In this study, we designed highly specific sensitive assay the using copper monosulfide (CuS) nanoparticles conjugated an anti-OTA antibody (CuS-Ab NPs) fluorescent probe Cu2+. When present in solution, antigen, bound microplate, competed off by soluble binding CuS-Ab NPs. After washing, NPs are removed. Subsequently, HCl added dissolve releasing Cu2+ activating probe. Thus, resultant fluorescence emission inversely proportional content solution. Under optimal conditions, detected 0.1–100 ng mL−1 with limit 0.01 mL−1. was tested corn, soybean, coffee samples, recoveries ranging 94% 110%. This strategy provides new approach mycotoxins other small-molecule analytes broad application potential safety quality control.